Contents:
Microbiology Laboratory: Basic Rules and Requirements 1.Pharmaceutical Microbiology and Basic Rules of Laboratory 2. Basic Requirements of Microbiology
Laboratory Instrumentation 3. Microscope 4.
Autoclave 5. Incubator 6. Hot Air Oven 7. Laminar Air Flow 8.
pH Meter 9. Spectrophotometer 10. Colony Counter Preparation of Culture Media, Sterilization and Culture Methods 11. Preparation and Sterilization of Media 12. Aseptic Technique and Transfer of Microorganisms 13. Growth Promotion Test of the Medium
14. Preparation of Agar Slants,
Stabs and Plates 15. Preservation of
Microbial Cultures Techniques for Isolation of Pure Culture from Mixed Culture 16. Streak Plate Method 17.
Pour Plate Method 18. Spread Plate
Technique Staining Techniques of Microorganisms 19. Preparation of a Bacterial Smear 20. Staining 21. Simple
Staining 22. Negative Staining 23. Gram Staining 24. Acid-Fast Staining 25.
Staining of Fungus by Lactophenol Cotton Blue Biochemical Tests for Identification of Bacteria 26. IMViC Tests 27.
Indole Test 28. Methyl Red and
Voges-Proskauer Test (MR-VP Test) 29.
Citrate Test 30. Carbohydrate
Fermentation Test 31. Starch
Hydrolysis Test 32. Gelatin
Hydrolysis Test 33. H2S Production
Test Bacterial Motility 34. Wet Mount Method 35.
Hanging Drop Method 36. Soft Agar
and Agar Deep Tube Methods Microbial Assay of Antibiotics 37. Microbial Assay of Antibiotics 38. Turbidimetric or Tube Assay Method Sterility Testing of Pharmaceuticals 39. Sterility Testing of Pharmaceuticals Environmental Monitoring 40. Environmental Monitoring by Settling Plate Method Bacterial Analysis of Water 41. Bacterial Examination of Water by Multiple Tube Fermentation Test
42. Bacteriological Examination of
Water by Membrane Filtration Method 43.
Analysis of Water for Total Bacterial Population by Standard Plate Count Microbial Taxonomy
44. Fungi 45. Bacteria |
About the Authors:
Dr. G .Shyam Prasad is currently working as an Assistant professor of Microbiology, at
Vaagdevi Degree and PG College, Naimnagar, Hanamkonda, Warangal. He has 17
years of Teaching Research and Industrial experience. He received his Ph.D. in
2007 from Department of Microbiology, Kakatiya University, Warangal and he is
also awarded with CSIR-Research Associate ship, New Delhi, India. He is
also recepient of UGC-DR.D.S.Kothari post doctoral fellowship, New
Delhi, India (Department of Biochemistry, Osmania University, Hyderabad). He is
actively engaged in teaching to B-Pharmacy, BSc and MSc Sudents. He also guided
more than 12 graduation and post graduation projects. He is also serving as
Editorial Advisory board member of “International Journal of Pharmacy and
Biological sciences”. He is also Reviewer of more than 10 national and
International journals.He has filed 2 patents and written 3 book chapters and
published 26 research articles to his credit in various national and
international Journals. His research interests are in the area of
Biotransformation studies, Mycotoxins, Thermophilic fungi. He also collaborated
with Sonia M.Tiquia-Arashiro, Professor of Microbiology, University of
Michigan, US in writing a book.
Dr. K. Srisailam is Presently working
as Principal, University College of Pharmaceutical Sciences, Satavahana
University, Karimnagar. He has 17 years of teaching and research experience. He
has obtained his B.Pharm., M.Pharm. and Ph.D. from University College of
Pharmaceutical Sciences, Kakatiya University, Warangal. He was Qualified in
GATE 1998 and 1999 and Recipient of SRF from CSIR, New Delhi during Ph.D. He
also obtained PG Diploma in Intellectual Property Rights from IGNOU, New Delhi.
The various positions held are Subject Expert for GPAT, AICTE; ECET, Govt. of Andhra
Pradesh; PGECET, Government of Telangana. Subject Expert, Academic Staff
College, JNTU, Hyderabad. Guided more than 15 candidates for M. Pharm and 6
candidates for Ph.D. He is serving as Editor in Chief of Pharmacognosy
Research, Editor in Chief of Pharmacognosy Journal, Member, Editorial Board,
Journal of Pharmaceutical Negative Results. He is also serving as Reviewer for
about 40 international and 20 national journals. Currently, he has 4 book
chapters, 67 Publications in national and international journals. He has
affiliations to different organizations and Member of Organizing Committee of
several seminars and workshops. He was a Member, Board of Studies in Pharmacy,
Satavahana University, Karimnagar, Worked as Core Team
Member, Scientific Services Committee of 57th Indian Pharmaceutical Congress,
Worked in Pharma Alumni Association, UCPSc in various capacities from Executive
Member to General Secretary, Life Member, IPA, Society of Pharmacognosy, Indian
Pharmacological Society, International Society for Ethnopharmacology, APTI,
ISTE; and Registered Pharmacist, Andhra Pradesh State Pharmacy Council,
Hyderabad. |
(www.ulektz.com) 
